例句與造句

1. Refolding and high - level expression study on human interferon - in e . coli
   在大腸桿菌中高效表達和色譜復(fù)性研究
2. High - level expression of bydv gav coat protein gene in escherichia coli
   株系外殼蛋白基因在大腸桿菌中的高效表達
3. Construction of high - level expression strain of human acidic fibroblast growth factor
   人酸性成纖維細胞生長因子的高效表達
4. High - level expression and purification of recombinant staphylokinase from engineering bacterium
   重組葡激酶的高效表達與純化
5. Chemical synthesis of human interleukin - 18 gene and its high - level expression in e . coli
   人白細胞介素18基因化學(xué)合成及其在大腸桿菌中高表達
6. It's difficult to find high-level expression in a sentence. 用high-level expression造句挺難的
7. Strategies to achieve high - level expression of foreign genes in plants are mainly introduced in this paper
   摘要介紹提高外源基因在植物體內(nèi)表達的方法。
8. This set of objects might then serve as an intermediate result used in the processing of a higher - level expression
   這組對象可接著作為中間結(jié)果用于處理更高一級的表達式。
9. These include the ability to target the gene of interest to site - specific areas of the chloroplast genome , high - level expression of foreign genes and increased environment security
   由于植物葉綠體表達系統(tǒng)能夠高效表達目的基因、環(huán)境安全性高，已成為目前植物生物反應(yīng)器研究的熱點之一。
10. After getting the cdna sequence of curcin , four fragments of the gene were cloned through pcr and high - level expression in e . coli was achieved . the four target dna fragments , i . e
    在得到麻瘋樹毒蛋白cdna序列后，通過pcr對毒蛋白基因進行了克隆，并實現(xiàn)了部分基因片段在大腸桿菌中的高效表達。
11. Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible
    結(jié)論成功構(gòu)建了echistatin的原核高效表達克隆，表達量高于現(xiàn)有國內(nèi)外研究水平，為echistatin功能及相關(guān)疾病的研究奠定了基礎(chǔ)。
12. Governmental information protection policy is a high - level expression of strategic objectives for protecting governmental information , it is not the same as the more detailed information security of contingency policy for the government
    摘要政府信息保護政策是為了保護政府的信息在策略性目標的一種高層次的表達方式，它與政府的精密信息安全或突發(fā)事件政策是不同。
13. Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing
    目的構(gòu)建蛇毒鋸鱗蝰素( echistatin )的原核高效表達體系方法由genebank數(shù)據(jù)庫檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列，結(jié)合大腸桿菌蛋白質(zhì)合成體系對氨基酸密碼子使用的偏愛性，設(shè)計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當(dāng)?shù)南拗菩詢?nèi)切酶位點插入表達載體pbv220 ，分別構(gòu)建了echistatin的單拷貝表達克隆、雙拷貝串聯(lián)表達克?。贿M一步通過pcr技術(shù)構(gòu)建echistatin的融合表達基因克隆。
14. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l . , pichia methanolica high - level expression systems of the genes have been constructed , and the milligram expressed protein was purified using probond resin purification system , which may result in further identification of the function of the aba binding protein . the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr , 369 bp long 3 ' - utr and poly ( a ) tail . the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr , 144 bp long 3 " - utr and poly ( a ) tail
    3 - 5 - race擴增片段序列分析結(jié)果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區(qū)為876個核苷酸， 3非翻譯區(qū)為369個核苷酸并末端帶poly ( a )尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架( 2304bp ) ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區(qū)為88個核苷酸， 3非翻譯區(qū)為144個核苷酸并末端帶poly ( a )尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架( 780bp ) 。
15. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium , then , induced by 0 . 5 % methanol . the expression protein was analyzed by sds - page after five days of induction . sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately , account for 30 % and 10 % of the total protein separately , which were purified using probond resin purification system , and obtained 15mg at levels above 0 . 75g / l and 7mg expression protein at levels above 0 . 35g / l separately once purification , the purity is both above 90 %
    篩選ade +表型轉(zhuǎn)化子， 20mlbmmy搖瓶培養(yǎng)，用0 . 5甲醇誘導(dǎo)表達5天后， sds - page檢測結(jié)果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經(jīng)過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
16. However , the quantities of foreign gene products are far lower than that of the original polyhedra . in this study , we explored functional mechanism of polyhedron gene sequence on expression level of hbsag ( pres2 + s ) genes and optimized the conditions for high - level expression in silkworm - bmnpv expression vector system
    為了進一步提高外源蛋白在家蠶bmnpv表達系統(tǒng)中的表達水平，我們以乙肝病毒表面抗原( pres2 + s )為研究對象，探討了多角體基因序列對外源蛋白在家蠶中表達的作用及其作用機制，并對該基因高效表達的環(huán)境條件進行了優(yōu)化。
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